Chromosoom 8 en PCR


Chromosome 8 is one of the 23 pairs of chromosomes in humans. People normally have two copies of this chromosome. Chromosome 8 spans about 145 million base pairs (the building material of DNA) and represents between 4.5 and 5.0% of the total DNA in cells. About 8% of its genes are involved in brain development and function, and about 16% are involved in cancer. A unique feature of 8p is a region of about 15 megabases that appears to have a high mutation rate. This region shows a significant divergence between human and chimpanzee, suggesting that its high mutation rates have contributed to the evolution of the human brain.

One of the WHO primer sequences in the PCR test for SARS-CoV-2 is found in all human DNA. The sequence ‘CTCCCTTTGTTGTGTTGT’ is an 18-character primer sequence found in the WHO coronavirus PCR testing protocol document. The primer sequences are what get amplified by the PCR process in order to be detected and designated a ‘positive’ test result. This exact same 18-character sequence, is also found on Homo sapiens chromosome 8. This means that the WHO test kits should find a positive result in all humans.

In summary, although the sequence CTCCCTTTGTTGTGTTGT is present in the human chromosome 8, only one of the two primers used in the test for SARS-CoV-2 is able to recognize this sequence. The test would therefore always produce a negative result if only human DNA was present. Therefore the claim that the RT-PCR test would produce a false-positive result due to the presence of human DNA—and the implication that the number of positive SARS-CoV-2 test results is artificially inflated for this reason—is incorrect and misleading.

Human DNA does not produce a positive result on the RT-PCR test for SARS-CoV-2

Furthermore, it is evident from testing statistics that not all tests produce positive results; according to the U.S. Centers for Disease Control and Prevention’s COVID Data Tracker, only 9% of all tests conducted in the U.S. are positive as of 30 August 2020, as Lead Stories pointed out in their fact-check of the same blog post:

Fact Check: WHO Tests For Coronavirus Do NOT Automatically Deliver False Positive Every Time

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